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3-ZoneReagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
Key features of 3-ZoneReagent include:
• Permits the isolation of RNA, DNA, and protein from the same sample
• Offers superior lysis capability, even with difficult sample types
• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
Reliably purify RNA from multiple sample volumes and sources
3-ZoneReagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with protocols for purification from samples of human, animal, plant, or bacterial origin. 3-ZoneReagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the 3-ZoneReagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by 3-ZoneReagent is free of protein and DNA contamination.
Formulated for isolation of multiple molecular targets
3-ZoneReagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with 3-ZoneReagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
|Downstream Application:||Cloning,Northern Blotting,Nuclease Protection Assays,Real-Time Quantitative PCR (qPCR),Reverse Transcriptase PCR (RT-PCR),cDNA Library Construction|
|Final Product:||Total RNA,Transcriptome RNA,micro RNA|
|High Throughput Compatibility:||Not High Throughput-Compatible (Manual)|
|Isolation Technology:||Organic Extraction|
|Number of Reactions:||100 Preps|
|Product Size:||100 mL|
|Purification Time:||1 hr|
|Sample Type (General):||Bacteria,Blood,Cells,Plant Samples,Tissue,Viral Samples,Yeast|
|Shipping Condition:||Room Temperature|
|Starting Material (Amount):||Up to 1 g tissue,Up to 1 x 10^7 cells|
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