HiFi Taq PCR Core KIT

The PCR Core Kit is designed to determine individual PCR conditions for all basic PCR applications relying on Taq DNA Polymerase. Assays which might give better sensitivity and specificity with a non-standard Mg-concentration in the reaction buffer can be optimized using the Mg-free buffer supplied with this kit.

Opis

The PCR Core Kit is designed to determine individual PCR conditions for all basic PCR applications relying on Taq DNA Polymerase. Assays which might give better sensitivity and specificity with a non-standard Mg-concentration in the reaction buffer can be optimized using the Mg-free buffer supplied with this kit.

High Fidelity HiFi Taq DNA Polymerase is an enzyme blend of recombinant Pfu and recombinant Taq DNA Polymerase with an efficient proofreading 3’ – 5’ exonuclease activity for improved PCR reactions. High Fidelity Taq DNA Polymerase is an efficient enzyme mixture that greatly increases fidelity and amplification of genomic targets up to 10 kb with high specificity, sensitivity, accuracy, and yield.

Advantages 

  • Proofreading 3’ – 5’ exonuclease activity
  • High Fidelity
  • Recombinant sourced Taq DNA Polymerase
  • Amplification: genomic targets up to 10 kb
  • Storage: -20ºC for extended periods
  • Shipping: 2-8ºC (dry ice is not required)
  • 3′ – 5′ Exonuclease Proofreading Ability: YES
  • 5′ – 3′ Exonuclease Activity: YES

Applications

Full-length Gene Amplification, Gene Mutation Detection, L-PCR, PCR-ELISA

Source

HiFi Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition

One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.

Quality Control

The quality of HiFi Taq DNA Polymerase is tested on a lot-to-lot basis according to Novazym quality management system. PCR is used to ensure consistent amplification of genomic targets using HiFi Taq DNA Polymerase.

Kit components:

  • Reaction buffer  with Mg2+ (10x): 700 mM Tris-HCl pH 8.6 / 25 °C, 166 mM (NH4)2SO4, 25 mM MgCl2
  • Reaction buffer  without Mg2+ (10x): 700 mM Tris-HCl pH 8.6 / 25 °C, 166 mM (NH4)2SO4
  • Storage buffer 10x): 20 mM Tris-HCl pH 8.0, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% NonidetTM P40, 0.5% TweenTM 20, 50% glicerol.
  • 25 mM MgCl2
  • dNTPs

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