HiFi Taq DNA polymerase

HiFi Taq DNA Polymerase is an enzyme blend of recombinant Pfu and recombinant Taq DNA Polymerase with an efficient proofreading 3’ – 5’ exonuclease activity for improved PCR reactions. High Fidelity Taq DNA Polymerase is an efficient enzyme mixture that greatly increases fidelity and amplification of genomic targets up to 10 kb with high specificity, sensitivity, accuracy, and yield.

Advantages 

• Proofreading 3’ – 5’ exonuclease activity
• High Fidelity
• Recombinant sourced Taq DNA Polymerase
• Amplification: genomic targets up to 10 kb
• Storage: -20ºC for extended periods
• Shipping: 2-8ºC (dry ice is not required)
• 3′ – 5′ Exonuclease Proofreading Ability: YES
• 5′ – 3′ Exonuclease Activity: YES

Applications

Full-length Gene Amplification, Gene Mutation Detection, L-PCR, PCR-ELISA

Source

HiFi Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition

One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.

Quality Control

The quality of HiFi Taq DNA Polymerase is tested on a lot-to-lot basis according to Novazym quality management system. PCR is used to ensure consistent amplification of genomic targets using HiFi Taq DNA Polymerase.