Fluorescently labelled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. We applied a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.

Short name Full name Excitation wavelengths Emission wavelengths
5-FAM 5-Carboxyfluorescein 492 nm 517 nm
6-FAM 6-Carboxyfluorescein 492 nm 517 nm
Abz Anthranilyl, 2-Aminobenzoyl 320 nm 420 nm
Cy3 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride 550 nm 570 nm
Cy5 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride 650 nm 670 nm
Dansyl 5-(Dimethylamino) naphthalene-1-sulfonyl 342 nm 562 nm
EDANS 5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid 340 nm 490 nm
FITC Fluorescein isothiocyanate 490 nm 520 nm
Mca 7-Methoxycoumarinyl-4-acetyl 325 nm 392 nm
Rhodamine B [9-(2-carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium chloride 553 nm 627 nm
5-TAMRA Carboxytetramethyl rhodamine 543 nm 572 nm


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