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Opis
Fluorescently labelled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. We applied a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.
Short name | Full name | Excitation wavelengths | Emission wavelengths |
5-FAM | 5-Carboxyfluorescein | 492 nm | 517 nm |
6-FAM | 6-Carboxyfluorescein | 492 nm | 517 nm |
Abz | Anthranilyl, 2-Aminobenzoyl | 320 nm | 420 nm |
Cy3 | 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride | 550 nm | 570 nm |
Cy5 | 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride | 650 nm | 670 nm |
Dansyl | 5-(Dimethylamino) naphthalene-1-sulfonyl | 342 nm | 562 nm |
EDANS | 5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid | 340 nm | 490 nm |
FITC | Fluorescein isothiocyanate | 490 nm | 520 nm |
Mca | 7-Methoxycoumarinyl-4-acetyl | 325 nm | 392 nm |
Rhodamine B | [9-(2-carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium chloride | 553 nm | 627 nm |
5-TAMRA | Carboxytetramethyl rhodamine | 543 nm | 572 nm |
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