Aflatoxin M1 Rapid Test

Aflatoxin M1 Aspergillus fungi mycotoxin Rapid detection Kit.

Aflatoxin M1 is formed as the metabolite of other aflatoxins in animals’ organisms., including cattle, that are fed with the forage infected with aflatoxins. That is why, they can be present in the cows’ milk. For the harmful activity of that aflatoxins especially small children are exposed. Aflatoxin M1 is a chromatographic immunoassay kit for rapid detection of aflatoxin M1. The method is based on immunocompetence ELISA test. The binding of the test substance to the receptor surface binding site is examined to detect the mycotoxin in sample. Different colour changes indicate different concentrations. This technology has high specificity, sensitivity, strong stability and anti-interference ability. The more mycotoxins in the sample, the less fluorescent-labelled antibodies is binding to the test line. The detection limit is 0,05 ppb, 0,4 ppb or 0,5 ppb depending on the test kind.

MATERIALS PROVIDED

• Test Strip, 40 pcs in 5 bottles, 8 pcs/bottle
• Diluent buffer, 16 ml
• Microwells with reagent – 5 pcs
• Manual , 1pc
• Reader (optional)

SPECIMEN COLLECTION AND STORAGE

1. Raw, well mixed milk without precipitates and clots.
2. Sample should have room temperature.

TEST PROCEDURE

1. Place all specimens, test devices, and allow them to room temperature prior to testing.
2. Remove the reagent bucket from the original packaging, then open it, remove the required number of microwell reagents and test strips and mark them. Please, use it as soon as possible within 60 min. Immediately after removing the test reagent, cover the lid.
3. Pipette around 200 µl of prepared milk sample to microwells. Slowly aspirate and mix well with reagents in the microwells.
4. After incubating for 5 min at room temperature (20-25 °C), insert the labeled test strip into the microwell with the end with ‘MAX’ sign. Allow it to fully immerse into the solution.
5. After incubating for 5 minutes at room temperature (20-25 °C) again, take out the strip and judge according to the schematic diagram.
6. The results can be analyzed by standard way or using the reader.

INTERPRETATION OF THE RESULTS

QUALITATIVE READING

POSITIVE: T line color is the same as C line, T line color is weaker than C line or C line is colored and T line is not colored at all. That means, that the concentration of aflatoxin B1 in the sample is equal to or higher, than the detection limit.

NEGATIVE: Both, the C and T lines are colored and the T line is stronger than the C line. That indicates, that the concentration of aflatoxin in the sample is below the detection limit.

INVALID: The C line does not appear, indicating the incorrect operation process or strip deterioration. In this case, read the instructions carefully and retest with a new strip. If the test strip needs to be recorded, please cut off the lower sponge pad immediately after the interpretation and dry it for archiving.