Aflatoxin B1 Rapid Test

Aflatoxin B1 Aspergillus fungi mycotoxin Rapid detection Kit.

Aflatoxin B1 is produced by Aspergillus fungi. Those toxins are mutagenic and teratogenic. Aflatoxin B1 is the most common from this type of toxins. Its acceptable amount is 2 µg/kg. Aflatoxin B1 is a chromatographic immunoassay kit for rapid detection of aflatoxin B1. The method is based on immunocompetence ELISA test. The binding of the test substance to the receptor surface binding site is examined to detect the mycotoxin in sample. Different color changes indicate different concentrations. This technology has high specificity, sensitivity, strong stability and anti-interference ability. The more mycotoxins in the sample, the less fluorescent-labelled antibodies is binding to the test line. The detection limit is 5-8 ppb.

MATERIALS PROVIDED

• Test Strip, 40 pcs in 5 bottles, 8 pcs/bottle
• Diluent buffer, 16 ml
• Microwells with reagent – 5 pcs
• Manual , 1pc
• Reader (optional)

MATERIALS REQUIRED, BUT NOT PROVIDED

• Anhydrous methanol
• Equipment: centrifuge, crusher, tube for the sample, pipette

SPECIMEN COLLECTION AND STORAGE

1. Weigh 5g of ground cereals to the 50 ml tube and add 25 ml of methanol.
2. Mix the sample for 1-2 min, then centrifuge for 3 minutes in 6000 rpm.
3. Mix 100 µl of supernatant with 400 µl of the buffer present in the kit.

TEST PROCEDURE

1. Place all specimens, test devices, and allow them to room temperature prior to testing.
2. Remove the reagent bucket from the original packaging, then open it, remove the required number of microwell reagents and test strips and mark them. Please, use it as soon as possible within 60 min. Immediately after removing the test reagent, cover the lid.
3. Pipette 200 µl to microwells. Slowly aspirate and mix well with reagents in the microwells.
4. After incubating for 5 min at room temperature (20-25 °C), insert the labeled test strip into the microwell with the end with ‘MAX’ sign. Allow it to fully immerse into the solution.
5. After incubating for 5 minutes at room temperature (20-25 °C) again, take out the strip and judge according to the schematic diagram.
6. The results can be analyzed by standard way or using the reader.

INTERPRETATION OF THE RESULTS

QUALITATIVE READING

POSITIVE: T line color is the same as C line, T line color is weaker than C line or C line is colored and T line is not colored at all. That means, that the concentration of aflatoxin B1 in the sample is equal to or higher, than the detection limit.

NEGATIVE: Both, the C and T lines are colored and the T line is stronger than the C line. That indicates, that the concentration of aflatoxin in the sample is below the detection limit.

INVALID: The C line does not appear, indicating the incorrect operation process or strip deterioration. In this case, read the instructions carefully and retest with a new strip. If the test strip needs to be recorded, please cut off the lower sponge pad immediately after the interpretation and dry it for archiving.