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Chemically synthesized peptides have free N-terminus. Via N-terminal acetylation the charge is removed from the amino terminus of a peptide. Generally, acetyl modification is recommended to make the peptide more closely mimic the charge state in the native protein. In addition this modification stabilizes the resulting peptide, and enhances their ability to resist enzymatic degradation by exo-peptidases.
A multitude of N-terminal modifications can be applied by Novazym’s peptide synthesis experts. Frequently applied N-terminal modifications are listed in the table below.
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