Silica 3-ZoneReagent is a complete, ready-to-use KIT for fast purification of high-quality total RNA or the simultaneous isolation of RNA variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
Key features of Kit include:
• Permits fast isolation of RNA, • Offers superior lysis capability, even with difficult sample types • Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
Reliably purify RNA from multiple sample volumes and sources Silica 3-ZoneReagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with protocols for purification from samples of human, animal, plant, or bacterial origin. Silica 3-Zone Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the Silica 3-Zone Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed less then half hour. Total RNA isolated by Silica 3-ZoneReagent is free of protein and DNA contamination.
Formulated for isolation of multiple molecular targets Silica 3-ZoneReagent allows you to perform sequential precipitation of RNAs from a single sample. After homogenizing the sample with Silica 3-ZoneReagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with 70% isopropanol. RNA can be precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation.