|Allegro Taq PLUS DNA Polymerase is a thermostable DNA polymerase. Due to its genetic modifications Allegro Taq has an enhanced stability. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity. Enzyme is available in concentration 10u/ul.|
Gene Amplification, qPCR, nested PCR
Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.
One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.
The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Novazym quality management system. PCR is used to ensure consistent amplification of genomic targets using Taq DNA Polymerase.