5x NOVAMaster Mix ready-to-load (RT stable, dye)

5x NOVAMaster Mix is a premixed ready-to-load solution containing all reagents (dye included) required for PCR (except template, primers and water).  We recommend using 5x NOVAMaster Mix in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. Due to its genetic modifications NOVA Taq  has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

Advantages 

• Highly processive,
• Exonuclease activity 5’ – 3’
• Recombinant sourced Taq DNA Polymerase
• Amplification: genomic targets up to 6kb
• Storage: room temperature  for extended periods
• Shipping: room temperature  (dry ice,coolers are not required)
• available in mixes with: 7,5mM or 12,5mM MgCl₂

Applications

Full-length Gene Amplification, Gene Mutation Detection, L-PCR, PCR-ELISA

Source

Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition

One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.

Quality Control

The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Novazym quality management system. PCR is used to ensure consistent amplification of genomic targets using Taq DNA Polymerase.

The highest quality Novazym product.