VivaTaq PCR Core KIT

Description

The PCR Core Kit is designed to determine individual PCR conditions for all basic PCR applications relying on Taq DNA Polymerase. Assays which might give better sensitivity and specificity with a non-standard Mg-concentration in the reaction buffer can be optimized using the Mg-free buffer supplied with this kit.

VivaTaq DNA Polymerase is a hot-start, thermostable DNA polymerase. Due to its genetic modifications VivaTaq  has an enhanced stability at room temperature with no activity up to 55C. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.Enzyme is only available in concentration 5u/u and 10u/ul.

Advantages 

• hot-start “aptamer blocking”
• Exonuclease activity 5’ – 3’
• Highly processive,
• Recombinant sourced Taq DNA Polymerase
• Amplification: genomic targets up to 6kb
• Storage: RT for extended periods
• Shipping: RT (dry ice is not required)
• 5′ – 3′ Exonuclease Activity: YES

Applications

Gene Amplification, qPCR, nested PCR

Kit components:

• Reaction buffer  with Mg2+ (10x): 700 mM Tris-HCl pH 8.6 / 25 °C, 166 mM (NH4)₂SO₄, 25 mM MgCl₂
• Reaction buffer  without Mg2+ (10x): 700 mM Tris-HCl pH 8.6 / 25 °C, 166 mM (NH4)₂SO₄
• Storage buffer 10x): 20 mM Tris-HCl pH 8.0, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% NonidetTM P40, 0.5% TweenTM 20, 50% glicerol.
• 25 mM MgCl₂
• dNTPs

Source

Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition

One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.

Quality Control

The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Novazym quality management system. PCR is used to ensure consistent amplification of genomic targets using Taq DNA Polymerase.