Description

Allegro Taq DNA Polymerase is a thermostable DNA polymerase. Due to its genetic modifications Allegro Taq has an enhanced stability. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity. Enzyme is available in concentration 5u/u.

Advantages

Standard application Taq Polymerase
Highly processive,
Recombinant source
Amplification: genomic targets up to 6kb
Storage:-20⁰C for extended periods
Shipping: 4⁰C (dry ice is not required!)
5′ – 3′ Exonuclease Activity: YES

Concentration: 5u/ul

Applications

Gene Amplification, qPCR, nested PCR

Source

Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition

One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.

Quality Control

The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Novazym quality management system. PCR is used to ensure consistent amplification of genomic targets using Taq DNA Polymerase.

Allegro Taq DNA polymerase (5u/ul)