Viva Taq DNA polymerase

VivaTaq DNA Polymerase is a hot-start, thermostable DNA polymerase. Due to its genetic modifications VivaTaq  has an no activity up to 55C. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.Enzyme is only available in concentration 5u/u.

Advantages 

• hot-start activity
• Exonuclease activity 5’ – 3’
• Highly processive,
• Recombinant sourced Taq DNA Polymerase
• Amplification: genomic targets up to 6kb
• 5′ – 3′ Exonuclease Activity: YES

Applications

Gene Amplification, qPCR, nested PCR

Source

Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition

One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.

Quality Control

The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Novazym quality management system. PCR is used to ensure consistent amplification of genomic targets using Taq DNA Polymerase.